TiD is a standalone application, which relies on basic assumption that a protein must be essential for pathogens survival and non-homologous with host to qualify as putative target. With an input bacterial proteome, TiD removes paralogous proteins, picks essential ones, and excludes proteins homologous with host organisms. The targets illustrate non-homology with at least 40 out of 84 gut microbes,
considered safe for human. TiD classifies proposed targets as known, novel and virulent. Users can perform pathway analysis, choke point
analysis, interactome analysis, subcellular localization and functional annotations through web servers cross-referenced with the application.
Drug targets identified by TiD for Listeria monocytogenes, Bacillus anthracis and Pseudomonas aeruginosa have revealed significant overlaps
with previous studies. TiD takes less than two hours to scan putative targets from a bacterial proteome with ~5000 proteins; hence, we propose
it as a useful tool for rational drug design.